Active Recombinant Human TGM2 Protein, His-tagged

• Cat.No.: TGM2-628H
• Product Overview: Recombinant Human TGM2 (Ala2-Ala687, Asn533Thr and Leu655Val), with an N-terminal Met and 6-His tag was expressed in Spodoptera frugiperda, Sf 21 (baculovirus).

Specification

• Description: Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene.
• Source: Insect cells
• Species: Human
• Tag: His
• Bio-activity: Measured by its ability to cleave a synthetic peptide Benzyloxycarbonyl-Gln-Gly and NH2OH. The specific activity is >1000 pmol/min/μg, as measured under the described condition.
• Molecular Mass: Predicted Molecular Mass: 78 kDa
  SDS-PAGE: 82 kDa, reducing conditions
• Protein length: Ala2-Ala687
• Endotoxin: < 1.0 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Applications: Enzyme Activity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT.
• Shipping: The product is shipped with dry ice or equivalent.

Gene Information

• Gene Name: TGM2 transglutaminase 2 [ Homo sapiens (human) ]
• Official Symbol: TGM2
• Synonyms: TGM2; transglutaminase 2; TGC; tTG; G(h); hTG2; TG(C); protein-glutamine gamma-glutamyltransferase 2; C polypeptide, protein-glutamine-gamma-glutamyltransferase; TGase C; TGase H; TGase II; TGase-2; erythrocyte transglutaminase; heart G alpha(h); hhG alpha(h); isopeptidase TGM2; protein G alpha(h); protein-glutamine deamidase TGM2; protein-glutamine dopaminyltransferase TGM2; protein-glutamine histaminyltransferase TGM2; protein-glutamine noradrenalinyltransferase TGM2; protein-glutamine serotonyltransferase TGM2; tTgase; tissue transglutaminase; transglutaminase C; transglutaminase H; transglutaminase II; EC 2.3.2.13; EC 3.5.1.44
• Gene ID: 7052
• mRNA Refseq: NM_198951
• Protein Refseq: NP_945189
• MIM: 190196
• UniProt ID: P21980