G-Actin/F-actin In Vivo Assay Biochem Kit

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G-Actin/F-actin In Vivo Assay Biochem Kit

G-Actin/F-actin Related Products

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Cat.No. : Kit-0354
Product Overview : The most reproducible and accurate method of determining the amount of filamentous actin (F-actin) content versus free globular-actin (G-actin) content in a cell population is to use Western blot quantitation of F-actin and G-actin cellular fractions. The general approach is to homogenize cells in F-actin stabilization buffer, followed by centrifugation to separate the F-actin from G-actin pool. The fractions are then separated by SDS-PAGE and actin is quantitated by Western blot. The final result gives the most accurate method of determining the ratio of F-actin incorporated into the cytoskeleton versus the G-actin found in the cytosol. This kit contains all the reagents needed to perform this assay.
Size : 30-100 assays
Applications : 1. To study the effects of pharmaceutical compounds on the ratio of G-actin to F-actin.2. To study the effects of mutated cell lines versus their parent cell line for the change in ratio of G-actin to F-actin.3. To study the effects of physical alterations of environment on the ratio of G-actin to F-actin.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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