Glucose Uptake Colorimetric Assay Kit

• Cat.No.: Kit-2249
• Product Overview: Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells. In glucose uptake colorimetric assay kit, the 2-DG6P is oxidized to generate NADPH, which can be determined by an enzymatic recycling amplification reaction. This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well.

Specification

• Tag: Non
• Applications: This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well.
• Storage: -20°C
• Shipping: Gel Pack
• Size: 100 assays
• Kit Components: Extraction Buffer; Neutralization Buffer; 2-Deoxyglucose (2-DG, 10 mM); Assay Buffer; Enzyme Mix (lyophilized); Recycling Mix (lyophilized); 2-DG6P Standard (lyophilized); Glutathione Reductase (lyophilized); Substrate-DTNB (lyophilized)
• Target Species: Mammalian
• Detection method: Absorbance (412 nm)
• Features & Benefits: Simple procedure;
  Fast and convenient;
  The assay is easy, non-radioactive, and highly sensitive