"Cell" Related Products

Live/Dead Cell Viability Assay Kit (for Mammalian Cells)

Cat.No.: Kit-1014
Description: Quantification of number of live and dead cells is an indispensable tool in cell biology research. Live/Dead Cell Viability Assay Kit, provides a two-color fluorescence method that is based on the simultaneous determination of live and dead cells using two different dyes. Live cell dye easily penetrates intact,; Live cells and intracellular esterase hydrolyzes the dye to produce a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm which can be measured at Ex/Em = 485/530 nm. Dead cell dye enters damaged cell membranes and undergoes a 40-fold enhancement of fluorescence upon binding to nucleic acid, thereby producing a bright red fluorescence (Ex/Em = 495/635 nm) in dead cells. This assay kit provides an easy-to-use, non-radioactive, histological and FACS-based method for measuring cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.
Applications: Screening/studying/characterization of stimulators/inhibitors that affect cell viability.
Storage: -20°C
Shipping: Gel Pack
Size: 100 assays
Kit Components: Assay Buffer; Live Cell Staining Dye; Dead Cell Staining Dye
Target Species: Mammalian
Detection method: Fluorescent microscopy or FACS analysis (Ex/Em485/530 nm) to detect stained live cells; (Ex/Em 495/635) to detect stained dead cells
Features & Benefits: Easy to use Protocol
Non-radioactive detection. Detect using either microscopy or FACS.
Measure Cell Proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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