Recombinant Bovine TMPRSS15 Protein, His-tagged TMPRSS15-02H

Recombinant Bovine TMPRSS15 Protein, His-tagged

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Recombinant Bovine TMPRSS15 Protein, His-tagged

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Cat.No. : TMPRSS15-02H
Product Overview : Recombinant bovine TMPRSS15 protein (801-1,035) with a C-termina His-tag enterokinase was expressed in yeast.
Description : Enteropeptidase is a membrane-bound serine protease that converts the inactive enzyme trypsinogen to the active form trypsin, a protease that catalyzes the digestion of proteins in the gut. It is composed of an N-terminal domain with a transmembrane segment that anchors enteropeptidase to the cell membrane and an extracellular C-terminal protease domain that contains the activation cleavage site for enteropeptidase activity and a catalytic aspartic acid-histidine-serine triad. It is synthesized in the endoplasmic reticulum as a zymogen and transported to the brush border membrane of duodenal and jejunal enterocytes. Enteropeptidase activation occurs in a calcium- and pH-dependent manner and, upon activation, cleaves the Asp-Asp-Asp-Asp-Lys activation peptide on trypsinogen to produce trypsin. Pharmacological inhibition of enteropeptidase activity decreases food intake, body weight gain, and liver triglyceride and total cholesterol levels in diet-induced obese mice and diabetic obese ob/ob mice.
Source : Yeast
Species : Bovine
Tag : His
Form : Lyophilized from sterile 10 mM Tris-HCl, pH 7.2, with 2 mM calcium chloride, 100 mM sodium chloride, and 50% glycerol
Molecular Mass : 27.1 kDa
Protein length : 801-1035
Purity : ≥95% estimated by SDS-PAGE
Stability : ≥ 1 year
Storage : Store at -80 centigrade
SDS-PAGE Analysis of Enteropeptidase :

Lane 1: MW Markers, Lane 2: Enteropeptidase, SDS-PAGE Analysis of Enteropeptidase. This protein has a calculated molecular weight of 27.1 kDa. It has an apparent molecular weight of approximately 44 kDa by SDS-PAGE under reducing conditions due to glycosylation.

Gene Name : TMPRSS15 transmembrane serine protease 15 [ Bos taurus (cattle) ]
Official Symbol : TMPRSS15
Synonyms : TMPRSS15; transmembrane serine protease 15; PRSS7; enteropeptidase; enterokinase; protease, serine, 7 (enterokinase); serine protease 7; transmembrane protease, serine 15
Gene ID : 282009
mRNA Refseq : NM_174439
Protein Refseq : NP_776864
UniProt ID : P98072

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