Recombinant Bovine TMPRSS15 Protein, His-tagged

• Cat.No.: TMPRSS15-02H
• Product Overview: Recombinant bovine TMPRSS15 protein (801-1,035) with a C-termina His-tag enterokinase was expressed in yeast.

Specification

• Description: Enteropeptidase is a membrane-bound serine protease that converts the inactive enzyme trypsinogen to the active form trypsin, a protease that catalyzes the digestion of proteins in the gut. It is composed of an N-terminal domain with a transmembrane segment that anchors enteropeptidase to the cell membrane and an extracellular C-terminal protease domain that contains the activation cleavage site for enteropeptidase activity and a catalytic aspartic acid-histidine-serine triad. It is synthesized in the endoplasmic reticulum as a zymogen and transported to the brush border membrane of duodenal and jejunal enterocytes. Enteropeptidase activation occurs in a calcium- and pH-dependent manner and, upon activation, cleaves the Asp-Asp-Asp-Asp-Lys activation peptide on trypsinogen to produce trypsin. Pharmacological inhibition of enteropeptidase activity decreases food intake, body weight gain, and liver triglyceride and total cholesterol levels in diet-induced obese mice and diabetic obese ob/ob mice.
• Source: Yeast
• Species: Bovine
• Tag: His
• Form: Lyophilized from sterile 10 mM Tris-HCl, pH 7.2, with 2 mM calcium chloride, 100 mM sodium chloride, and 50% glycerol
• Molecular Mass: 27.1 kDa
• Protein length: 801-1035
• Purity: ≥95% estimated by SDS-PAGE
• Stability: ≥ 1 year
• Storage: Store at -80 centigrade

Gene Information

• Gene Name: TMPRSS15 transmembrane serine protease 15 [ Bos taurus (cattle) ]
• Official Symbol: TMPRSS15
• Synonyms: TMPRSS15; transmembrane serine protease 15; PRSS7; enteropeptidase; enterokinase; protease, serine, 7 (enterokinase); serine protease 7; transmembrane protease, serine 15
• Gene ID: 282009
• mRNA Refseq: NM_174439
• Protein Refseq: NP_776864
• UniProt ID: P98072