Recombinant Full Length Human PDHA1 Protein, C-Flag-tagged

• Cat.No.: PDHA1-1646HFL
• Product Overview: Recombinant Full Length Human PDHA1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 40.2 kDa
• AA Sequence: MRKMLAAVSRVLSGASQKPASRVLV ASRNFANDATFEIKKCDLHRLEEGP PVTTVLTREDGLKYYRMMQT VRRMELKADQLYKQKIIRGFCHLCD GQEACCVGLEAGINPTDHLITAYRA HGFTFTRGLSVREILAELTG RKGGCAKGKGGSMHMYAKNFYGGNG IVGAQVPLGAGIALACKYNGKDEVC LTLYGDGAANQGQIFEAYNM AALWKLPCIFICENNRYGMGTSVER AAASTDYYKRGDFIPGLRVDGMDIL CVREATRFAAAYCRSGKGPI LMELQTYRYHGHSMSDPGVSYRTRE EIQEVRSKSDPIMLLKDRMVNSNLA SVEELKEIDVEVRKEIEDAA
  QFATADPEPPLEELGYHIYSSDPPF EVRGANQWIKFKSVSTRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine biosynthesis

Gene Information

• Gene Name: PDHA1 pyruvate dehydrogenase E1 subunit alpha 1 [ Homo sapiens (human) ]
• Official Symbol: PDHA1
• Synonyms: PDHA; PDHAD; PHE1A; E1alpha; PDHCE1A
• Gene ID: 5160
• mRNA Refseq: NM_000284.4
• Protein Refseq: NP_000275.1
• MIM: 300502
• UniProt ID: P08559