Recombinant Full Length Salmonella Dublin Probable 4-Amino-4-Deoxy-L-Arabinose-Phosphoundecaprenol Flippase Subunit Arnf(Arnf) Protein, His-Tagged

• Cat.No.: RFL20446SF
• Product Overview: Recombinant Full Length Salmonella dublin Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF(arnF) Protein (B5FPE2) (1-125aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Salmonella dublin (strain CT_02021853)
• Tag: His
• Form: Lyophilized powder
• Protein Length: Full Length (1-125)
• AA Sequence: MGVMWGLISVAIASLAQLSLGFAMM RLPSIAHPLAFISGLGALNAATLAL FAGLAGYLVS VFCWHKTLHTLALSKAYALLSLSYV LVWVASMLLPGLQGAFSLKAMLGVL CIMAGVMLIF LPARS
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: arnF
• Synonyms: arnF; SeD_A2647; Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF; L-Ara4N-phosphoundecaprenol flippase subunit ArnF; Undecaprenyl phosphate-aminoarabinose flippase subunit ArnF
• UniProt ID: B5FPE2

Gene Information

• Gene Name: arnF
• Synonyms: arnF; SeD_A2647; Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF; L-Ara4N-phosphoundecaprenol flippase subunit ArnF; Undecaprenyl phosphate-aminoarabinose flippase subunit ArnF
• UniProt ID: B5FPE2

Reviews

04/26/2023

In addition to the remarkable protein quality, the manufacturer provides excellent technical support, proving to be a valuable resource when troubleshooting experimental challenges.

06/25/2021

In addition to its outstanding quality, the manufacturer of the arnF protein provides excellent technical support, which is a valuable asset for researchers

12/09/2019

Its use in EM studies allows researchers to gain insights into the structural details of proteins and their complexes at high resolution.

03/28/2018

Their expertise and prompt assistance can effectively address any concerns or challenges that may arise during the experimental process.

11/20/2016

ts reliability and high quality make it a valuable tool in various applications, ranging from enzymatic assays to studying protein-protein interactions.

06/05/2016

the arnF protein is highly regarded for its excellent performance in WB assays and its significant contribution to protein EM structure analysis.

Q&As

Are there any known inhibitors or antagonists of the arnF protein?

While specific inhibitors or antagonists of the arnF protein have not been identified yet, some studies have explored compounds that target related proteins within the same biosynthetic pathway, which could indirectly affect the function of arnF.

How is the arnF protein synthesized within bacterial cells?

The arnF protein is synthesized through the process of translation, where the mRNA transcript of the arnF gene is translated into a sequence of amino acids, forming the protein.

Is the arnF gene conserved among different bacterial species?

The arnF gene can vary in sequence and presence among different bacterial species. However, it is often found in many Gram-negative bacteria that produce lipopolysaccharides.

How does the arnF protein contribute to bacterial drug resistance?

The arnF protein is part of the arnBCADTEF operon, which is involved in the modification of LPS. This modification helps the bacteria to resist the action of certain antibiotics, such as polymyxins, which target the bacterial cell membrane.

Does the arnF protein have any role in biofilm formation?

While the arnF protein is primarily associated with antibiotic resistance and lipopolysaccharide modification, there is emerging evidence suggesting its involvement in biofilm formation, a process by which bacteria can form a protective community on surfaces.

Are there any known genetic variations in the arnF gene within bacterial populations?

Yes, genetic variations, including point mutations or insertions and deletions, have been observed in the arnF gene among different bacterial strains. These variations can contribute to differences in drug resistance levels.

Are there any known structural features or domains of the arnF protein?

The arnF protein is a membrane protein that consists of transmembrane domains and extracellular loops. These structural features allow it to function in the modification of lipopolysaccharides.

Is the arnF gene constitutively expressed or regulated by specific promoter regions?

The expression of the arnF gene can be constitutive in some bacterial species, while in others, it can be regulated by specific promoter regions that respond to environmental cues or transcription factors.

Does the arnF protein have any connection to bacterial virulence?

While the arnF protein is primarily associated with antibiotic resistance, recent studies suggest its potential involvement in bacterial virulence. Further research is needed to establish a clear connection between the arnF protein and virulence mechanisms.

What are the implications of the arnF protein's involvement in antibiotic resistance?

The presence of the arnF protein and its role in modifying lipopolysaccharides can confer resistance to antibiotics like polymyxins. This can limit treatment options for bacterial infections and pose challenges in clinical settings.

Can the arnF protein contribute to bacterial adaptation to different environments?

The functionality of the arnF protein in modifying lipopolysaccharides may aid bacterial adaptation to different environments, including the ability to resist antibiotics, evade host immune responses, or survive in stressful conditions.

Are there any known mutations in the arnF gene associated with antibiotic resistance?

Yes, mutations in the arnF gene can lead to increased production of the arnF protein, resulting in enhanced resistance to polymyxins in some bacterial strains.

Can the arnF protein interact with other proteins within bacterial cells?

Yes, the arnF protein can interact with other proteins within bacterial cells, particularly those involved in the biosynthesis of lipopolysaccharides or antibiotic resistance mechanisms.

Does the arnF protein have any other known functions besides drug resistance?

The primary known function of the arnF protein is related to drug resistance. However, further research may uncover additional roles of this protein in bacterial physiology or pathogenicity.

Are there any known mechanisms by which bacteria can downregulate or inhibit the expression of arnF?

Bacteria may employ various mechanisms to downregulate or inhibit the expression of arnF, such as the presence of specific repressor proteins, regulatory RNAs, or epigenetic modifications that prevent its transcription or translation.

Are there any known homologs or related proteins to arnF in bacteria?

Yes, there are other proteins within the same operon or pathway, such as ArnB, ArnC, ArnD, ArnA, ArnT, and ArnE, that are involved in the biosynthesis of lipopolysaccharides and share functional similarities with arnF.

Can the expression of the arnF gene be influenced by environmental factors?

Yes, the expression of the arnF gene can be regulated by various environmental cues, such as the presence of antibiotics, stress conditions, or specific signaling molecules, which can induce or repress its expression.

Can the arnF protein undergo post-translational modifications?

Post-translational modifications, such as phosphorylation, acetylation, or glycosylation, may be possible for the arnF protein, but the specific modifications and their functional implications are not yet well-defined.

Can the arnF protein be a target for the development of new antibiotics?

The arnF protein and its involvement in antibiotic resistance mechanisms make it an attractive target for the development of new antibiotics that can inhibit its function or disrupt the modification of lipopolysaccharides.

Can the arnF protein be a target for developing new antibiotics?

Yes, targeting the arnF protein and the arnBCADTEF operon's biosynthetic pathway is an active area of research for developing novel antimicrobial agents that can disrupt bacterial resistance to polymyxins.