Recombinant HCoV-HKU1(isolate N1) Spike protein, His&Myc-tagged

• Cat.No.: Spike-4534H
• Product Overview: Recombinant HCoV-HKU1(isolate N1) Spike protein(Q5MQD0)(307-677aa), fused to N-terminal His tag and C-terminal Myc tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: HCoV-HKU1(isolate N1)
• Tag: His&Myc
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 46.5 kDa
• Protein length: 307-677aa
• AA Sequence: SGFTVKPVATVHRRIPDLPDCDIDK WLNNFNVPSPLNWERKIFSNCNFNL STLLRLVHTDSFSCNNFDESKIYGS CFKSIVLDKFAIPNSRRSDLQLGSS GFLQSSNYKIDTTSSSCQLYYSLPA INVTINNYNPSSWNRRYGFNNFNLS SHSVVYSRYCFSVNNTFCPCAKPSF ASSCKSHKPPSASCPIGTNYRSCES TTVLDHTDWCRCSCLPDPITAYDPR SCSQKKSLVGVGEHCAGFGVDEEKC GVLDGSYNVSCLCSTDAFLGWSYDT CVSNNRCNIFSNFILNGINSGTTCS NDLLQPNTEVFTDVCVDYDLYGITG QGIFKEVSAVYYNSWQNLLYDSNGN IIGFKDFVTNKTYNIFPCYAG
• Purity: Greater than 85% as determined by SDS-PAGE.
• Storage: Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C.