Recombinant Human AP3B1 293 Cell Lysate
- Gene Information
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|Description :||Antigen standard for adaptor-related protein complex 3, beta 1 subunit (AP3B1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.|
|Source :||HEK 293 cells|
|Components :||This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).|
|Size :||0.1 mg|
|Storage Instruction :||Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.|
|Applications :||ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.|
|Gene Name :||AP3B1 adaptor-related protein complex 3, beta 1 subunit [ Homo sapiens ]|
|Official Symbol :||AP3B1|
|Synonyms :||AP3B1; adaptor-related protein complex 3, beta 1 subunit; AP-3 complex subunit beta-1; ADTB3A; HPS2; beta3A-adaptin; beta-3A-adaptin; AP-3 complex beta-3A subunit; adaptor protein complex AP-3 subunit beta-1; adapter-related protein complex 3 subunit beta-1; clathrin assembly protein complex 3 beta-1 large chain; PE; HPS; ADTB3;|
|Gene ID :||8546|
|mRNA Refseq :||NM_003664|
|Protein Refseq :||NP_003655|
|UniProt ID :||O00203|
|Chromosome Location :||5q14.1|
|Pathway :||Clathrin derived vesicle budding, organism-specific biosystem; Golgi Associated Vesicle Biogenesis, organism-specific biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Membrane Trafficking, organism-specific biosystem; trans-Golgi Network Vesicle Budding, organism-specific biosystem;|
|Function :||binding; protein phosphatase binding; protein transporter activity;|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
The study of recombinant AP3B1 may help us better understand and treat diseases associated with abnormal intracellular vesicle transport.
The AP3B1 should be frozen at -80°C to ensure its long-term stability.
At present, there is no clear evidence that the AP3B1 has anti-tumor effect, but it may have a certain application prospect in the field of tumor therapy.
The recombinant AP3B1 protein can be used as a carrier to help transport drugs to specific organelles or tissues, thereby improving the efficiency of drug delivery.
AP3B1 can be expressed, extracted and purified in cells by genetic engineering technology.
The current study shows that the AP3B1 does not cause a significant immune response in most people.
Mutations in the AP3B1 gene can lead to an inherited neurodevelopmental disorder, AP-3 associated intracellular transport deficiency. AP3B1 research may contribute to the treatment and understanding of related diseases.
AP3B1 has potential application in disease treatment, drug delivery and other fields, but it is still in the research stage.
AP3B1 can bind to specific cell membrane proteins, and has certain targeting effects in vitro and in vivo.
The current study shows that the AP3B1 is safe at appropriate doses. Of course, further confirmation is needed before clinical application.
Customer Reviews (4)Write a review
The stability of the protein is tested by long-term storage or storage under different conditions, such as temperature, pH value, etc. -
The cytotoxicity of the protein is detected through the effect on cells to ensure the safety of the protein. -
The molecular weight of the protein and whether there are impurities were detected by SDS-PAGE, Western blot and other methods. -
The activity of protein, such as enzyme activity and binding activity, was detected by functional experiments. -
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