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Recombinant Human AP3B1 293 Cell Lysate

Cat.No. : AP3B1-8810HCL
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  • Gene Information
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Description : Antigen standard for adaptor-related protein complex 3, beta 1 subunit (AP3B1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : AP3B1 adaptor-related protein complex 3, beta 1 subunit [ Homo sapiens ]
Official Symbol : AP3B1
Synonyms : AP3B1; adaptor-related protein complex 3, beta 1 subunit; AP-3 complex subunit beta-1; ADTB3A; HPS2; beta3A-adaptin; beta-3A-adaptin; AP-3 complex beta-3A subunit; adaptor protein complex AP-3 subunit beta-1; adapter-related protein complex 3 subunit beta-1; clathrin assembly protein complex 3 beta-1 large chain; PE; HPS; ADTB3;
Gene ID : 8546
mRNA Refseq : NM_003664
Protein Refseq : NP_003655
MIM : 603401
UniProt ID : O00203
Chromosome Location : 5q14.1
Pathway : Clathrin derived vesicle budding, organism-specific biosystem; Golgi Associated Vesicle Biogenesis, organism-specific biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Membrane Trafficking, organism-specific biosystem; trans-Golgi Network Vesicle Budding, organism-specific biosystem;
Function : binding; protein phosphatase binding; protein transporter activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What is the association between recombinant AP3B1 and intracellular transport abnormalities? 12/21/2022

The study of recombinant AP3B1 may help us better understand and treat diseases associated with abnormal intracellular vesicle transport.

What are the long-term preservation conditions of AP3B1 ? 12/03/2022

The AP3B1 should be frozen at -80°C to ensure its long-term stability.

Does AP3B1 have anti-tumor effects? 11/28/2022

At present, there is no clear evidence that the AP3B1 has anti-tumor effect, but it may have a certain application prospect in the field of tumor therapy.

What is the relationship between recombinant AP3B1 and drug delivery? 04/27/2022

The recombinant AP3B1 protein can be used as a carrier to help transport drugs to specific organelles or tissues, thereby improving the efficiency of drug delivery.

How to prepare AP3B1 ? 04/15/2022

AP3B1 can be expressed, extracted and purified in cells by genetic engineering technology.

Is the recombinant AP3B1 immunogenic? 06/16/2021

The current study shows that the AP3B1 does not cause a significant immune response in most people.

What is the association between AP3B1 and genetic diseases? 08/08/2020

Mutations in the AP3B1 gene can lead to an inherited neurodevelopmental disorder, AP-3 associated intracellular transport deficiency. AP3B1 research may contribute to the treatment and understanding of related diseases.

What is the clinical application prospect of AP3B1 ? 11/10/2019

AP3B1 has potential application in disease treatment, drug delivery and other fields, but it is still in the research stage.

What is the targeting effect of AP3B1 ? 05/27/2019

AP3B1 can bind to specific cell membrane proteins, and has certain targeting effects in vitro and in vivo.

Is AP3B1 toxic? 01/21/2019

The current study shows that the AP3B1 is safe at appropriate doses. Of course, further confirmation is needed before clinical application.

Customer Reviews (4)

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Reviews
10/10/2022

    In the case of repeated experiments, the stability of this protein is still very good, indicating that it has high repeatability and reproducibility.

    02/04/2022

      Strip location in the Western blot was accurate and showed good specificity.

      11/21/2021

        Green production process of AP3B1 focuses on sustainable development and maximizes the utilization of energy and resources by optimizing the production process and equipment.

        01/17/2019

          The short half-life and high clearance properties of AP3B1 allow it to act quickly in the body and be cleared quickly, reducing the risk of side effects.

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