Recombinant Human AP3B1 Protein, His-tagged

• Cat.No.: AP3B1-188H
• Product Overview: Recombinant Human AP3B1 Protein (Met1-Leu233) with N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes a protein that may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. The encoded protein is part of the heterotetrameric AP-3 protein complex which interacts with the scaffolding protein clathrin. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 2. Two transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.5 kDa
  Accurate Molecular Mass: 30 kDa
• Protein length: Met1-Leu233
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: AP3B1 adaptor-related protein complex 3, beta 1 subunit [ Homo sapiens (human) ]
• Official Symbol: AP3B1
• Synonyms: AP3B1; adaptor-related protein complex 3, beta 1 subunit; AP-3 complex subunit beta-1; ADTB3A; HPS2; beta3A-adaptin; beta-3A-adaptin; AP-3 complex beta-3A subunit; adaptor protein complex AP-3 subunit beta-1; adapter-related protein complex 3 subunit beta-1; clathrin assembly protein complex 3 beta-1 large chain; PE; HPS; ADTB3
• Gene ID: 8546
• mRNA Refseq: NM_003664
• Protein Refseq: NP_003655
• MIM: 603401
• UniProt ID: O00203

Reviews

10/10/2022

In the case of repeated experiments, the stability of this protein is still very good, indicating that it has high repeatability and reproducibility.

02/04/2022

Strip location in the Western blot was accurate and showed good specificity.

11/21/2021

Green production process of AP3B1 focuses on sustainable development and maximizes the utilization of energy and resources by optimizing the production process and equipment.

01/17/2019

The short half-life and high clearance properties of AP3B1 allow it to act quickly in the body and be cleared quickly, reducing the risk of side effects.

Q&As

What is the association between recombinant AP3B1 and intracellular transport abnormalities?

The study of recombinant AP3B1 may help us better understand and treat diseases associated with abnormal intracellular vesicle transport.

What are the long-term preservation conditions of AP3B1 ?

The AP3B1 should be frozen at -80°C to ensure its long-term stability.

Does AP3B1 have anti-tumor effects?

At present, there is no clear evidence that the AP3B1 has anti-tumor effect, but it may have a certain application prospect in the field of tumor therapy.

What is the relationship between recombinant AP3B1 and drug delivery?

The recombinant AP3B1 protein can be used as a carrier to help transport drugs to specific organelles or tissues, thereby improving the efficiency of drug delivery.

How to prepare AP3B1 ?

AP3B1 can be expressed, extracted and purified in cells by genetic engineering technology.

Is the recombinant AP3B1 immunogenic?

The current study shows that the AP3B1 does not cause a significant immune response in most people.

What is the association between AP3B1 and genetic diseases?

Mutations in the AP3B1 gene can lead to an inherited neurodevelopmental disorder, AP-3 associated intracellular transport deficiency. AP3B1 research may contribute to the treatment and understanding of related diseases.

What is the clinical application prospect of AP3B1 ?

AP3B1 has potential application in disease treatment, drug delivery and other fields, but it is still in the research stage.

What is the targeting effect of AP3B1 ?

AP3B1 can bind to specific cell membrane proteins, and has certain targeting effects in vitro and in vivo.

Is AP3B1 toxic?

The current study shows that the AP3B1 is safe at appropriate doses. Of course, further confirmation is needed before clinical application.