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Recombinant Human ARF3 protein, GST-tagged

Cat.No. : ARF3-745H
Product Overview : Human ARF3 full-length ORF ( AAH07762, 1 a.a. - 181 a.a.) recombinant protein with GST-tag at N-terminal.
  • Specification
  • Gene Information
  • Related Products
Description : ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. The ARF3 gene contains five exons and four introns. [provided by RefSeq, Jul 2008]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 45.65 kDa
AA Sequence : MGNIFGNLLKSLIGKKEMRILMVGL DAAGKTTILYKLKLGEIVTTIPTIG FNVETVEYKNISFTVWDVGGQDKIR PLWRHYFQNTQGLIFVVDSNDRERV NEAREELMRMLAEDELRDAVLLVFA NKQDLPNAMNAAEITDKLGLHSLRH RNWYIQATCATSGDGLYEGLDWLAN QLKNKK
Applications : Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Gene Name : ARF3 ADP-ribosylation factor 3 [ Homo sapiens ]
Official Symbol : ARF3
Synonyms : ARF3; ADP-ribosylation factor 3; small GTP binding protein;
Gene ID : 377
mRNA Refseq : NM_001659
Protein Refseq : NP_001650
MIM : 103190
UniProt ID : P61204

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (20)

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Are there any therapeutic implications of targeting ARF3 protein? 04/17/2023

Targeting ARF3 protein or its associated pathways may have potential therapeutic implications, particularly in diseases characterized by abnormal vesicular trafficking, such as certain types of cancer, neurodegenerative disorders, and viral infections. However, further research is needed to fully explore these therapeutic possibilities.

How is ARF3 protein involved in cellular homeostasis? 03/10/2023

ARF3 protein contributes to cellular homeostasis by regulating intracellular trafficking and maintaining the proper distribution of proteins and lipids within cells. By ensuring the correct delivery of molecules to their target compartments, ARF3 helps in maintaining cellular integrity and the functioning of organelles.

Are there any known protein-protein interactions of ARF3? 02/21/2023

ARF3 protein can interact with various proteins to carry out its functions. Some known protein-protein interactions include interactions with coat proteins (such as COPI and clathrin), cargo adaptors/receptors (such as AP-1, GGAs, and SNXs), SNARE proteins, and regulators of membrane fusion (such as rab effectors and tethering factors).

Are there any genetic mutations associated with ARF3 protein dysfunction? 12/02/2022

While rare, some genetic mutations in the ARF3 gene have been identified in individuals with developmental disorders or neurological conditions, emphasizing the importance of ARF3 protein in proper cellular functioning.

How does ARF3 protein contribute to cargo sorting in vesicle trafficking? 08/12/2022

ARF3 protein interacts with cargo receptors or adaptors within the Golgi apparatus or endosomes, mediating the sorting and packaging of specific cargoes into vesicles destined for their respective target compartments.

Are there any known ARF3 inhibitors or activators? 05/22/2022

While there are currently no specific inhibitors or activators for ARF3 protein available for clinical use, several compounds and small molecules have been developed for research purposes that can modulate ARF3 activity or its associated pathways. These molecules can be utilized as tools to investigate the cellular functions of ARF3 and may have future therapeutic applications.

Are there any inhibitors or activators specifically targeting ARF3 protein? 01/16/2022

Yes, small molecule inhibitors and activators targeting ARF3 protein or its associated pathways have been developed for research purposes, potentially aiding in drug discovery efforts targeted towards vesicular trafficking-related diseases.

Are there any diseases associated with ARF3 protein dysfunction? 01/05/2022

While rare, dysfunction in ARF3 protein has been implicated in certain genetic and neurodegenerative disorders, highlighting its importance in maintaining proper cellular trafficking pathways.

What are the upstream regulators of ARF3 protein? 07/07/2021

The activation of ARF3 protein can be regulated by various upstream regulators. Small GTPases from the ARF family, such as ARF guanine nucleotide exchange factors (ARFGEFs), facilitate the exchange of GDP for GTP on ARF3, leading to its activation. Additionally, other signaling pathways, such as the phosphoinositide 3-kinase (PI3K) pathway, can also regulate ARF3 activity.

Can ARF3 protein be targeted for therapeutic interventions? 01/31/2021

While targeting ARF3 directly may be challenging, modulating its activity or associated pathways could have therapeutic implications. For example, targeting ARFGEFs or ARF3 downstream effectors could potentially alter ARF3-mediated vesicular trafficking and be utilized in the treatment of diseases where this process is dysregulated.

Can ARF3 protein regulate membrane fusion events? 10/30/2020

Yes, ARF3 protein can regulate membrane fusion events by interacting with SNARE proteins and other regulators involved in vesicle tethering and fusion processes.

Can ARF3 protein interact with other cellular signaling pathways? 06/12/2020

Yes, ARF3 protein can interact with various signaling pathways, including the PI3K-Akt pathway and the Rho GTPase signaling network, to coordinate cellular processes like cell proliferation, survival, and cytoskeletal rearrangements.

Are there any disease associations with ARF3 protein dysfunction? 04/23/2020

Although not extensively studied, dysregulation of ARF3 protein has been implicated in certain diseases. For example, altered ARF3 expression and activity have been observed in neurodegenerative diseases like Alzheimer's and Parkinson's, suggesting a potential role in their pathogenesis.

How does ARF3 protein contribute to organelle biogenesis? 12/12/2019

ARF3 protein is involved in the formation and maintenance of various organelles. By regulating membrane trafficking and vesicle budding, ARF3 helps in the biogenesis of organelles such as the Golgi apparatus, endosomes, and secretory vesicles.

Does ARF3 protein have any roles in neuronal function? 11/11/2019

Yes, ARF3 protein has been implicated in synaptic vesicle recycling, axonal transport, and dendritic spine morphogenesis, suggesting its involvement in regulating neuronal function and synaptic plasticity.

Can ARF3 protein be inactivated? 06/09/2019

Yes, ARF3 protein can be inactivated by GTPase-activating proteins (GAPs), which stimulate the hydrolysis of GTP to GDP, returning ARF3 to its inactive state.

What are the specific effectors of ARF3 protein? 03/16/2019

Some effectors of ARF3 protein include COPI and clathrin adaptor proteins, which assist in coat assembly during vesicle formation, as well as phospholipase D, which participates in membrane remodeling events.

How is ARF3 protein regulated during cellular stress? 02/07/2019

Cellular stress conditions, such as oxidative stress or nutrient deprivation, can modulate ARF3 protein activity. Various stress-responsive signaling pathways, including the MAPK pathway, can regulate ARF3 activation or inhibit its function to adapt cellular responses and maintain cell viability under stress.

How does ARF3 protein work? 12/11/2017

ARF3 protein functions as a molecular switch by cycling between an active GTP-bound form and an inactive GDP-bound form. In its active state, ARF3 recruits various effectors and regulators to modulate vesicle trafficking processes.

Does ARF3 play a role in cytokinesis? 07/02/2016

Yes, ARF3 protein has been implicated in cytokinesis, the process of cell division. It is involved in the regulation of membrane trafficking events required for the constriction of the contractile ring and the formation of the cleavage furrow during cytokinesis.

Customer Reviews (8)

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Reviews
08/29/2022

    Whether I need expertise in experimental optimization or troubleshooting, I can rely on the manufacturer's support to promptly address any concerns or queries.

    03/28/2022

      Its purity and reliability ensure reliable and reproducible results in a variety of research applications.

      03/19/2022

        the manufacturer's support team provides extensive technical assistance, helping researchers navigate any challenges they may face throughout their experiments.

        10/04/2020

          ARF3 protein is known for its versatility and functionality, enabling researchers to explore various biological processes and molecular interactions.

          03/07/2019

            Its versatility and reliability make it a great choice for researchers exploring protein dynamics, function, and interactions.

            09/07/2016

              Its use in EM studies allows researchers to gain insights into the structural details of proteins and their complexes at high resolution.

              07/24/2016

                the ARF3 protein is highly regarded for its excellent performance in WB assays and its significant contribution to protein EM structure analysis.

                04/14/2016

                  The ARF3 protein exhibits outstanding performance in enzyme-linked immunosorbent assays (ELISA).

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