Recombinant Human ARFGAP3 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human ARFGAP3 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : ARFGAP3-575H
Product Overview : ARFGAP3 MS Standard C13 and N15-labeled recombinant protein (NP_055385) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 57 kDa
AA Sequence : MGDPSKQDIL TIFKRLRSVP TNKVCFDCGA KNPSWASITY GVFLCIDCSG SHRSLGVHLS FIRSTELDSN WSWFQLRCMQ VGGNASASSF FHQHGCSTND TNAKYNSRAA QLYREKIKSL ASQATRKHGT DLWLDSCVVP PLSPPPKEED FFASHVSPEV SDTAWASAIA EPSSLTSRPV ETTLENNEGG QEQGPSVEGL NVPTKATLEV SSIIKKKPNQ AKKGLGAKKG SLGAQKLANT CFNEIEKQAQ AADKMKEQED LAKVVSKEES IVSSLRLAYK DLEIQMKKDE KMNISGKKNV DSDRLGMGFG NCRSVISHSV TSDMQTIEQE SPIMAKPRKK YNDDSDDSYF TSSSRYFDEP VELRSSSFSS WDDSSDSYWK KETSKDTETV LKTTGYSDRP TARRKPDYEP VENTDEAQKK FGNVKAISSD MYFGRQSQAD YETRARLERL SASSSISSAD LFEEPRKQPA GNYSLSSVLP NAPDMAQFKQ GVRSVAGKLS VFANGVVTSI QDRYGSTRTR PLEQKLISEE DLAANDILDY KDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : ARFGAP3 ADP-ribosylation factor GTPase activating protein 3 [ Homo sapiens (human) ]
Official Symbol : ARFGAP3
Synonyms : ARFGAP3; ADP-ribosylation factor GTPase activating protein 3; ADP ribosylation factor GTPase activating protein 1, ARFGAP1; ADP-ribosylation factor GTPase-activating protein 3; ARF GAP 3; ADP-ribosylation factor GTPase activating protein 1; ARFGAP1; FLJ45618;
Gene ID : 26286
mRNA Refseq : NM_014570
Protein Refseq : NP_055385
MIM : 612439
UniProt ID : Q9NP61

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