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Recombinant Human ARFGAP3 Protein, Myc/DDK-tagged, C13 and N15-labeled

Cat.No. : ARFGAP3-575H
Product Overview : ARFGAP3 MS Standard C13 and N15-labeled recombinant protein (NP_055385) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
  • Specification
  • Gene Information
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Description : The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 57 kDa
AA Sequence : MGDPSKQDILTIFKRLRSVPTNKVC FDCGAKNPSWASITYGVFLCIDCSG SHRSLGVHLSFIRSTELDSNWSWFQ LRCMQVGGNASASSFFHQHGCSTND TNAKYNSRAAQLYREKIKSLASQAT RKHGTDLWLDSCVVPPLSPPPKEED FFASHVSPEVSDTAWASAIAEPSSL TSRPVETTLENNEGGQEQGPSVEGL NVPTKATLEVSSIIKKKPNQAKKGL GAKKGSLGAQKLANTCFNEIEKQAQ AADKMKEQEDLAKVVSKEESIVSSL RLAYKDLEIQMKKDEKMNISGKKNV DSDRLGMGFGNCRSVISHSVTSDMQ TIEQESPIMAKPRKKYNDDSDDSYF TSSSRYFDEPVELRSSSFSSWDDSS DSYWKKETSKDTETVLKTTGYSDRP TARRKPDYEPVENTDEAQKKFGNVK AISSDMYFGRQSQADYETRARLERL SASSSISSADLFEEPRKQPAGNYSL SSVLPNAPDMAQFKQGVRSVAGKLS VFANGVVTSIQDRYGSTRTRPLEQK LISEEDLAANDILDYKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
Gene Name : ARFGAP3 ADP-ribosylation factor GTPase activating protein 3 [ Homo sapiens (human) ]
Official Symbol : ARFGAP3
Synonyms : ARFGAP3; ADP-ribosylation factor GTPase activating protein 3; ADP ribosylation factor GTPase activating protein 1, ARFGAP1; ADP-ribosylation factor GTPase-activating protein 3; ARF GAP 3; ADP-ribosylation factor GTPase activating protein 1; ARFGAP1; FLJ45618;
Gene ID : 26286
mRNA Refseq : NM_014570
Protein Refseq : NP_055385
MIM : 612439
UniProt ID : Q9NP61

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (15)

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Are there any inhibitors or modulators that target ARFGAP3? 09/18/2021

Currently, there are no known specific inhibitors or modulators developed specifically for ARFGAP3. However, small molecules or compounds that target the ARF-ARFGAP system in general may indirectly affect ARFGAP3 activity.

Does ARFGAP3 interact with other proteins? 06/05/2021

Yes, ARFGAP3 interacts with several proteins involved in membrane trafficking and remodeling processes. These include ARF family GTPases, SNARE proteins, clathrin, and various adaptor proteins. These interactions play a role in vesicle formation, cargo sorting, and membrane fusion events.

Are there any known interactions or binding partners for ARFGAP3? 04/08/2021

Yes, ARFGAP3 interacts with various proteins involved in vesicle trafficking and membrane dynamics. For example, it has been found to associate with coatomer protein complex I (COPI) subunits, cytoskeleton-related proteins, and lipid-modifying enzymes such as phospholipase D1 (PLD1). These interactions suggest its involvement in the regulation of vesicle formation and lipid metabolism.

Are there any known physiological functions of ARFGAP3? 10/02/2020

ARFGAP3 is involved in various physiological processes, including intracellular vesicle trafficking, membrane remodeling, Golgi organization, and regulated secretion. It also plays a role in cell migration, adhesion, and cytokinesis. The precise functions of ARFGAP3 can vary depending on the specific cell type and context.

Can ARFGAP3 regulate protein sorting and trafficking? 10/01/2019

Yes, ARFGAP3 is involved in protein sorting and trafficking processes within the cell. It functions as a GTPase-activating protein (GAP) for ARF GTPases, which regulate the formation of coat protein complexes involved in vesicle budding and cargo selection. By controlling the activity of ARF GTPases, ARFGAP3 influences the dynamics of vesicular trafficking.

Can ARFGAP3 influence cancer progression? 08/17/2018

There is limited research on the role of ARFGAP3 in cancer. However, some studies suggest that ARFGAP3 may be involved in cancer cell migration and invasion, indicating its potential contribution to cancer progression. Further investigation is required to fully understand the implications of ARFGAP3 in cancer biology.

How does ARFGAP3 contribute to vesicle trafficking? 05/18/2018

ARFGAP3 plays a role in COPI vesicle formation, which is involved in retrograde transport from the Golgi to the endoplasmic reticulum. It promotes the disassembly of COPI coats from transport vesicles, allowing cargo release and subsequent vesicle fusion.

Are there any experimental tools or methods to study ARFGAP3 function? 02/01/2018

Yes, researchers use several approaches to study ARFGAP3 function. These include gene knockout or knockdown techniques in cell lines or model organisms to investigate the phenotypic effects of ARFGAP3 loss. Immunofluorescence, live-cell imaging, and biochemical assays are also used to examine protein localization, interactions, and activity. Additionally, in vitro reconstitution experiments and structural analyses provide insights into the mechanistic details of ARFGAP3 function.

Are there any known genetic disorders associated with mutations in ARFGAP3? 12/02/2017

Currently, mutations in ARFGAP3 have been linked to autosomal recessive spastic paraplegia type 49 (SPG49). SPG49 is a rare neurodegenerative disorder characterized by progressive lower limb weakness and spasticity. However, more research is needed to fully understand the genetic landscape associated with ARFGAP3 mutations.

Is ARFGAP3 associated with any signaling pathways? 06/09/2017

While ARFGAP3 is primarily known for its role in membrane trafficking, it has been implicated in various signaling pathways as well. For example, ARFGAP3 may interact with components of the phosphoinositide 3-kinase (PI3K) signaling pathway, affecting downstream signaling events. However, further research is needed to fully elucidate the extent and mechanisms of ARFGAP3 involvement in signaling pathways.

What are the physiological functions of ARFGAP3? 05/27/2017

ARFGAP3 is critical for maintaining proper protein trafficking and Golgi function. It influences processes such as Golgi organization, endocytic trafficking, and protein secretion. Additionally, studies have implicated ARFGAP3 in cellular processes like cell adhesion and migration.

Are there any known alternative splicing variants of ARFGAP3? 03/04/2017

Yes, alternative splicing of the ARFGAP3 gene leads to the generation of different isoforms. These isoforms may possess distinct structural and functional properties, allowing for specificity in their cellular roles.

Is ARFGAP3 unique to humans, or is it found in other organisms as well? 07/02/2016

ARFGAP3 is evolutionarily conserved and found in various organisms, including mammals, birds, amphibians, and some invertebrates. Its presence across different species suggests its importance in fundamental cellular processes.

Does ARFGAP3 have any role in membrane curvature regulation? 03/20/2016

Yes, ARFGAP3 has been implicated in membrane curvature regulation, specifically in the context of forming tubular structures during membrane remodeling and vesicle trafficking processes. It interacts with proteins involved in shaping membranes and may contribute to membrane curvature through these interactions.

Is there any disease association with ARFGAP3? 01/21/2016

Some research suggests that ARFGAP3 mutations may be associated with certain neurological disorders, such as autosomal recessive spastic paraplegia. However, further studies are needed to fully understand the implications of ARFGAP3 in disease.

Customer Reviews (8)

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Reviews
09/20/2021

    Its exceptional properties and reliable results make it a valuable tool in various experimental applications.

    07/04/2021

      Its remarkable attributes and consistent results make it an indispensable tool in various experimental applications.

      04/21/2021

        By utilizing the ARFGAP3 protein, researchers can confidently explore the intricate details of protein structures and gain invaluable insights into their functionality.

        12/03/2019

          Highly recommended and known for its outstanding performance in ELISA, the argO protein is a top choice for researchers in protein electron microscopy structure analysis.

          11/05/2019

            Its remarkable specificity and functionality have allowed me to investigate various cellular processes with precision and confidence.

            09/08/2019

              With their assistance, I have been able to maximize the potential of the ARFGAP3 Protein and achieve even better results.

              03/02/2019

                the manufacturer's support has been instrumental in assisting me throughout my work with the ARFGAP3 protein.

                01/18/2018

                  Its exceptional quality ensures accurate data and supports the validity of my research findings.

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