| Description : |
This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation. |
| Source : |
Baculovirus-Insect Cells |
| Species : |
Human |
| Tag : |
C-His |
| Bio-activity : |
Measured by its ability to convert the substrate inosine 5' phosphate (IMP) to xanthosine 5' phosphate (XMP). The specific activity is 136 pmol/min/μg as measured under the described conditions. |
| Molecular Mass : |
The protein has a calculated MW of 57 kDa. |
| AA Sequence : |
MADYLISGGTSYVPDDGLTAQQLFN CGDGLTYNDFLILPGYIDFTADQVD LTSALTKKITLKTPLVSSPMDTVTE AGMAIAMALTGGIGFIHHNCTPEFQ ANEVRKVKKYEQGFITDPVVLSPKD RVRDVFEAKARHGFCGIPITDTGRM GSRLVGIISSRDIDFLKEEEHDCFL EEIMTKREDLVVAPAGITLKEANEI LQRSKKGKLPIVNEDDELVAIIART DLKKNRDYPLASKDAKKQLLCGAAI GTHEDDKYRLDLLAQAGVDVVVLDS SQGNSIFQINMIKYIKDKYPNLQVI GGNVVTAAQAKNLIDAGVDALRVGM GSGSICITQEVLACGRPQATAVYKV SEYARRFGVPVIADGGIQNVGHIAK ALALGASTVMMGSLLAATTEAPGEY FFSDGIRLKKYRGMGSLDAMDKHLS SQNRYFSEADKIKVAQGVSGAVQDK GSIHKFVPYLIAGIQHSCQDIGAKS LTQVRAMMYSGELKFEKRTSSAQVE GGVHSLHSYEKRLFHHHHHHHHHH |
| Endotoxin : |
< 0.1 EU/μg by LAL |
| Purity : |
> 90% by SDS-PAGE |
| Storage : |
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Concentration : |
0.33 mg/mL by Bradford |
| Storage Buffer : |
Sterile 20 mM Tris, 0.5M NaCl, 10% glycerol, 2mM DTT, pH 8.0 |
