Recombinant Human MRPS25 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human MRPS25 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : MRPS25-5894H
Product Overview : MRPS25 MS Standard C13 and N15-labeled recombinant protein (NP_071942) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 20.1 kDa
AA Sequence : MPMKGRFPIR RTLQYLSQGN VVFKDSVKVM TVNYNTHGEL GEGARKFVFF NIPQIQYKNP WVQIMMFKNM TPSPFLRFYL DSGEQVLVDV ETKSNKEIME HIRKILGKNE ETLREEEEEK KQLSHPANFG PRKYCLRECI CEVEGQVPCP SLVPLPKEMR GKYKAALKAD AQDTRTRPLE QKLISEEDLA ANDILDYKDD DDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : MRPS25 mitochondrial ribosomal protein S25 [ Homo sapiens (human) ]
Official Symbol : MRPS25
Synonyms : MRPS25; mitochondrial ribosomal protein S25; MRP-S25;
Gene ID : 64432
mRNA Refseq : NM_022497
Protein Refseq : NP_071942
MIM : 611987
UniProt ID : P82663

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