Recombinant Full Length Human SRC Protein, C-Flag-tagged

• Cat.No.: SRC-407HFL
• Product Overview: Recombinant Full Length Human SRC Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 59.7 kDa
• AA Sequence: MGSNKSKPKDASQRRRSLEPAENVH GAGGGAFPASQTPSKPASADGHRGP SAAFAPAAAEPKLFGGFNSS DTVTSPQRAGPLAGGVTTFVALYDY ESRTETDLSFKKGERLQIVNNTEGD WWLAHSLSTGQTGYIPSNYV APSDSIQAEEWYFGKITRRESERLL LNAENPRGTFLVRESETTKGAYCLS VSDFDNAKGLNVKHYKIRKL DSGGFYITSRTQFNSLQQLVAYYSK HADGLCHRLTTVCPTSKPQTQGLAK DAWEIPRESLRLEVKLGQGC FGEVWMGTWNGTTRVAIKTLKPGTM SPEAFLQEAQVMKKLRHEKLVQLYA VVSEEPIYIVTEYMSKGSLL DFLKGETGKYLRLPQLVDMAAQIAS GMAYVERMNYVHRDLRAANILVGEN LVCKVADFGLARLIEDNEYT ARQGAKFPIKWTAPEAALYGRFTIK SDVWSFGILLTELTTKGRVPYPGMV NREVLDQVERGYRMPCPPEC
  PESLHDLMCQCWRKEPEERPTFEYL QAFLEDYFTSTEPQYQPGENLTRTR PLEQKLISEEDLAANDILDYKDDDD KV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Stem cell relevant signaling - JAK/STAT signaling pathway
• Protein Pathways: Adherens junction, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, GnRH signaling pathway, Tight junction, VEGF signaling pathway

Gene Information

• Gene Name: SRC SRC proto-oncogene, non-receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: SRC
• Synonyms: ASV; SRC1; THC6; c-SRC; p60-Src
• Gene ID: 6714
• mRNA Refseq: NM_005417.5
• Protein Refseq: NP_005408.1
• MIM: 190090
• UniProt ID: P12931