Thiol Fluorometric Detection Kit Kit-0816

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Cat.No. :	Kit-0816
Product Overview :	Thiol Fluorometric Detection Kit provides an ultrasensitive fluorimetric assay to quantitate thiol content that exists either in a small molecule or on a protein. The proprietary non-fluorescent dye used in the kit becomes strongly fluorescent upon reacting with thiol. The kit can detect as little as 1 picomole of cysteine or GSH in a 100 µL assay volume (10 nM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. The thiol sensor used in the kit generates a strongly fluorescent adduct upon reacting with a thiol compound. The resulted adduct has the spectral proterties almost identical to those of fluorescein. In addition, both absorption and emission spectra of the thiol adduct are pH-independent, making this assay kit highly robust. The signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/520 nm.
Publication :	An aminotransferase is responsible for the deamination of the N-terminal leucine and required for formation of oxazolone ring A in methanobactin of Methylosinus trichosporium OB3b. (2017)

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