1,5-Anhydroglucitol Cell-Based Uptake Assay Kit

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1,5-Anhydroglucitol Cell-Based Uptake Assay Kit

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Cat.No. : Kit-2115
Product Overview : 1,5-Anhydroglucitol Uptake Assay Kit utilizes a proprietary fluorescent 1,5-AG analog, which can be taken up by cells. However, this 1,5-AG analog cannot be fully utilized in glycolytic processes and thus accumulates inside the cells. Fluorescence generated by this analog is proportional to the cellular 1,5-AG uptake. The assay can be used to monitor 1,5-AG uptake by using fluorescent microscopy and flow cytometry. Phloretin, a compound that can inhibit glucose transporter and 1,5-AG cell uptake, is included in the kit as a control. This non-radioactive assay kit is easy-to-use and allows qualitative and quantitative measurements of 1,5-AG uptake in cultured cells.
Size : 50 assays
Applications : Measurement of 1,5-Anhydroglucitol uptake in response to insulin, growth factors, cytokines, mitogens and nutrients, etc.Dual-staining of 1,5-AG transporters and 1,5-AG uptake Analysis of 1,5-AG metabolism and cell signaling in various cell typesScreening of anti-diabetic compounds
Storage : Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.Analysis Buffer: Ready to use. Keep on ice while in use. Store at -20 °C.AGTracker Reagent (100X): Aliquot and store at -20°C. For consistent results, avoid repeated freeze/thaw.Phloretin (100X): Bring to room temperature. Ready to use. Store at -20°C.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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