Measurement of glucose uptake in response to insulin, growth factors, cytokines, mitogens and nutrients, etc.Dual-staining of glucose transporters and glucose uptake Analysis of glucose metabolism and cell signaling in various cell types.Screening of anti-diabetic compounds.
Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.Analysis Buffer (50X): Dilute Analysis Buffer with 1X PBS (not supplied) to make 1X Analysis Buffer. Keep on ice while in use.2-NBDG Reagent (100X): Aliquot and store at -20⁰C. For consistent results, avoid repeated freeze/thaw.