Ac-DEVD-AFC

• Cat.No.: DYE-120

Specification

• Tag: Non
• Description: During apoptosis, activated caspase-3 cleaves several substrates, including poly (ADP-ribose) polymerase, which it specifically targets at the amino sequence DEVD. Ac-DEVD-AFC is a fluorogenic substrate for activated caspase-3 (Km = 9.7 μM), as well as related caspases. Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.
• Molecular Mass: 729.61
• Ex(nm): 351
• Em(nm): 430
• Solvent: DMSO
• Storage: Store at -20°C in the dark. Dessicated.