Acetyl Histone H4K16 Quantification Kit (Fluorometric)

• Cat.No.: Kit-0025
• Product Overview: Acetyl Histone H4K16 Quantification Kit (Fluorometric) is used for measuring acetylation of histone H4K16.

Specification

• Applications: Acetyl Histone H4K16 Quantification Kit (Fluorometric) is suitable for specifically measuring histone H4K16 acetylation using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, and cultured adherent and suspension cells.
• Storage: Upon receipt: (1) Store F4 and Standard Control at –20°C; (2) Store all other components at 4°C away from light. The components of the kit are stable for 6 months from the date of shipment, when stored properly.Note: Check if buffers F1 and F2 contain salt precipitates before using. If so, warm (at room temperature or 37°C) and shake the buffers until the salts are redissolved.

Reviews

03/17/2022

The protocol is simple and can be run in a short time.

11/02/2020

The kit is easy to use and I was able to get results in a short time.

06/09/2019

Follow the protocol. got great results.

Q&As

Histone acetylation mechanism?

Histone acetylation and deacetylation are essential parts of gene regulation. These reactions are typically catalysed by enzymes with "histone acetyltransferase" (HAT) activity. Acetylation is the process where an acetyl functional group is transferred from one molecule (in this case, acetyl coenzyme A) to another. Deacetylation is simply the reverse reaction where an acetyl group is removed from a molecule.Acetylation removes the positive charge on the histones, thereby decreasing the interaction of the N termini of histones with the negatively charged phosphate groups of DNA. As a consequence, the condensed chromatin is transformed into a more relaxed structure that is associated with greater levels of gene transcription.

Can samples flash frozen in liquid nitrogen and stored at -80˚C be tested?

Yes. But the activity from frozen tissues may be slightly lower than that obtained from fresh tissues.

What method/protocol can I use to quantify proteins/histones after the histone extraction procedure?

We recommend using any standard protein quantification assay such as a Bradford assay for histone quantification.

Can tissues or cell pellets be frozen prior to histone extraction?

Yes, it is possible for tissue or cell pellets to be frozen before extracting histone proteins.

What equipment will be used in the experiment?

You will need a fluorescent microplate reader and plate.

Can I use tissue samples lysed in RIPA buffer in my experiments?

RIPA buffer would not be compatible with the 10X Lysis Buffer. Please use the lysis buffer in the kit.