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Acetylcholinesterase Assay Kit

Cat.No. : Kit-2489
Product Overview : ACETYLCHOLINESTERASE (EC 3.1.1.7, AChE), also known as RBC cholinesterase, is found primarily in the blood and neural synapses. Low serum cholinesterase activity may relate to exposure to insecticides or to one of a number of variant genotypes. AChE catalyzes the hydrolysis of the neurotransmitter acetylcholine into choline and acetic acid, a reaction necessary to allow a cholinergic neuron to return to its resting state after activation. Cholinesterase levels of cells and plasma are used as a guide in establishing safety precautions relative to exposure and contact, as well as a guide in determining the need for workers to be removed from areas of contact with the organic phosphate insecticides. Simple, direct and automation-ready procedures for measuring AChE activity are very desirable. Acetylcholinesterase Assay is based on an improved Ellman method,in which thiocholine produced by the action of acetylcholinesterase forms a yellow color with 5,5’-dithiobis(2-nitrobenzoic acid). The intensity of the product color, measured at 412 nm, is proportionate to the enzyme activity in the sample.
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Storage : RT
Size : 100 tests

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (9)

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Is tetrahydrocannabinol an inhibitor of acetylcholinesterase? 11/24/2022

Yes.Tetrahydrocannabinol is a competitive inhibitor of acetylcholinesterase.

What are the drugs related to acetylcholinesterase? 03/10/2022

Drugs that reversibly inhibit acetylcholinesterase could be used as treatments for Alzheimer's disease and myasthenia gravis, among others. Examples include tacrine and donepezil.

Does acetylcholinesterase affect AD? 01/10/2021

AChE inhibitors have shown some benefit for Alzheimer disease. Inhibition of AChE results in a decreased breakdown and subsequent accumulation of acetylcholine. This excess acetylcholine leads to increased stimulation of muscarinic and nicotinic receptors, which provides some therapeutic relief for the memory deficits in AD.

What is the function of acetylcholinesterase? 11/14/2020

The primary role of AChE is to terminate neuronal transmission and signaling between synapses to prevent ACh dispersal and activation of nearby receptors. AChE is inhibited by organophosphates and is an important component of pesticides and nerve agents.

What is the sensitivity of the kit? 02/22/2020

Sensitivity: 10 U/L

How to measure the activity of AChE in whole blood? 10/01/2019

Whole blood and red blood cells (RBC) lysates can be prepared by diluting 1.0 ml of blood or RBC in 19.0 ml of 0.03% Triton X-100 in water. And then diluted in dilutions in Assay Buffer for further experiment.

Will ethanol inhibit the functionality of the assay? 01/15/2018

Studies have shown that ethanol can inhibit the activity of AChE.

Which lysis buffer should I use in order to keep the enzyme in active form? 11/19/2017

Mammalian Cell Lysis Buffer would be the best lysis buffer to use. <1% Chaps also is OK.

What sample types is this kit suitable for? 02/17/2017

Sample type: Cell culture extracts, Cell Lysate,Blood, Plasma, Serum, Tissue Lysate

Customer Reviews (3)

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Reviews
01/03/2023

    I am very satisfied with the results obtained with kit-2489.

    09/10/2022

      Product quality is guaranteed. I will continue to cooperate with Creative biomart.

      05/06/2021

        The product is precise and fast.

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