Acid Phosphatase Activity Colorimetric Assay Kit
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|Product Overview :||Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease. Acid Phosphatase Assay Kit is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax = 405 nm) when dephosphorylated by AP. The kit can detect μU acid phosphatase activity in samples.|
|Applications :||Phosphate concentrations between 1 μM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined.|
|Shipping :||Gel Pack|
|Size :||500 assays|
|Kit Components :||AP Assay Buffer; pNPP Substrate (10 TAB); AP Enzyme; Stop Solution|
|Target Species :||Mammalian|
|Detection method :||Absorbance (405 nm)|
|Features & Benefits :||
Simple procedure; takes ~ 3 hours;
Fast and convenient;
The assay is sensitive and stable;
The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.