Acid Phosphatase Activity Colorimetric Assay Kit


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Acid Phosphatase Activity Colorimetric Assay Kit

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Cat.No. : Kit-2280
Product Overview : Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease. Acid Phosphatase Assay Kit is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax = 405 nm) when dephosphorylated by AP. The kit can detect μU acid phosphatase activity in samples.
Applications : Phosphate concentrations between 1 μM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined.
Storage : -20°C
Shipping : Gel Pack
Size : 500 assays
Kit Components : AP Assay Buffer; pNPP Substrate (10 TAB); AP Enzyme; Stop Solution
Target Species : Mammalian
Detection method : Absorbance (405 nm)
Features & Benefits : Simple procedure; takes ~ 3 hours;
Fast and convenient;
The assay is sensitive and stable;
The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.

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