Acidic Sphingomyelinase Assay Kit (Fluorimetric) provides one of the most sensitive methods for detecting acidic SMase activity or screening its inhibitors. The kit uses AbRed as a fluorogenic probe to indirectly quantify the phosphocholine produced from the hydrolysis of sphingomyelin (SM) by sphingomyelinase (SMase). The fluorescence intensity of AbRed is proportional to the formation of phosphocholine, therefore to the SMase activity. It can be used for measuring the SMase activity in blood, cell extracts or other solutions. The kit is an optimized "mix and read" assay which is compatible with HTS liquid handling instruments.