Acitve Recombinant Human PRKAG1&PRKAB2&PRKAA2 protein(Met1-Pro331 & Met1-Ile270 & Met1-Arg552), His&GST-tagged

• Cat.No.: PRKAG1&PRKAB2&PRKAA2-1313H
• Product Overview: Recombinant Human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; Recombinant Human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; Recombinant Human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified and expreesed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-His&GST
• Protein length: Met1-Pro331 & Met1-Ile270 & Met1-Arg552
• Form: Supplied as sterile 50mM Tris, 200mM NaCl, 1mM EDTA, 1mM DTT, 0.5mM PMSF, 10% gly, 1mM GSH, pH 7.4.
• Bio-activity: The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substRate.
• Molecular Mass: The recombinant heterotrimer of Human AMPK (PRKAG1 / PRKAB2 / PRKAA2) has a calculated molecular mass of 158 (38+30+90) KDa. The apparent molecular mass is approximately 35, 37 & 95 KDa respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PRKAG1 protein kinase, AMP-activated, gamma 1 non-catalytic subunit [ Homo sapiens ]
• Official Symbol: PRKAG1; PRKAB2; PRKAA2
• Synonyms: PRKAG1; protein kinase, AMP-activated, gamma 1 non-catalytic subunit; 5-AMP-activated protein kinase subunit gamma-1; AMPK gamma1; AMPK gamma-1 chain; 5-AMP-activated protein kinase, gamma-1 subunit; AMPKG; MGC8666
• Gene ID: 5571
• mRNA Refseq: NM_001206709
• Protein Refseq: NP_001193638
• MIM: 602742
• UniProt ID: P54619