Acitve Recombinant Mouse PTPRC protein(Arg453-Ser1152), His&GST-tagged

• Cat.No.: PTPRC-1314M
• Product Overview: Recombinant Mouse PTPRC (AAA39458.1)(Arg453-Ser1152) was expreesed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Mouse
• Tag: N-His&GST
• Protein length: Arg453-Ser1152
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, 0.5mM GSH, 3mM DTT, pH 7.4, 10% gly.
• Bio-activity: 1. Measured by its ability to cleave a substRate, pNitrophenyl phosphate (pNPP). The specific activity is >50,000 pmoles/min/μg. 2. Measured by its ability to bind biotinylated Galectin-1 in a functional ELISA.
• Molecular Mass: The recombinant Mouse PTPRC/GST chimera consists of 937 amino acids and has a calculated molecular mass of 108.2 kDa. The recombinant protein migRates as an approximately 110 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ptprc protein tyrosine phosphatase, receptor type, C [ Mus musculus ]
• Official Symbol: PTPRC
• Synonyms: PTPRC; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; L-CA; lymphocyte antigen 5; leukocyte common antigen; lymphocyte common antigen; loc; B220; Cd45; Ly-5; T200; CD45R; Lyt-4
• Gene ID: 19264
• mRNA Refseq: NM_001111316
• Protein Refseq: NP_001104786