Acitve Recombinant Mouse PVR protein(Met1-Arg345), mFc-tagged

• Cat.No.: PVR-1294M
• Product Overview: Recombinant Mouse PVR (NP_081790.1) (Met1-Arg345) was expressed in HEK293 with the Fc region of Mouse IgG2a at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: mFc
• Protein Length: Met1-Arg345
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized TIGIT Protein, Human, Recombinant (ECD, His Tag) at 2 μg/ml (100 μl/well) can bind CD155/PVR Protein, Mouse, Recombinant (mFc Tag), the EC50 of CD155/PVR Protein, Mouse, Recombinant (mFc Tag) is 25-140 ng/mL. 2. Immobilized Mouse CD155 (mFc Tag) at 2 μg/mL (100μL/well) can bind Mouse TIGIT-His, HPLC-verified, the EC50 of Mouse TIGIT-His, HPLC-verified is 300-900 ng/mL.
• Molecular Mass: The recombinant Mouse PVR consists of 550 amino acids and predicts a molecular mass of 60.78 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Pvr poliovirus receptor [ Mus musculus ]
• Official Symbol: PVR
• Synonyms: PVR; poliovirus receptor; PVS; mE4; HVED; Taa1; CD155; Tage4
• Gene ID: 21332