Acitve Recombinant Rat IL23A & IL12B protein(Met1-Ala196 & Met1-Ser335), His-tagged
Cat.No. : | IL23A & IL12B-1231R |
Product Overview : | Recombinant Rat IL23A (NP_569094.1) (Met1-Ala196) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Rat IL12B (EDM04146.1) (Met1-Ser335) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Rat IL23A & the Rat IL12B heterodimer was purified and expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Rat |
Tag : | C-His |
Protein length : | Met1-Ala196 & Met1-Ser335 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured by its ability to induce IL17 secretion by Mouse splenocytes. The ED50 for this effect is 0.5-3ng/mL. |
Molecular Mass : | The recombinant Rat IL23A & the Rat IL12B comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.2 (21+37.2) KDa. The apparent molecular mass of the Rat IL23A & the Rat IL12B heterodimer is approximately 47.08, 43.29, 27.67 and 24.75 KDa respectively in SDS-PAGE. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 90 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | Il23a Interleukin 23, alpha subunit p19 [ Rattus norvegicus ] |
Official Symbol : | IL23A; IL12B |
Synonyms : | IL23A; Interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin-23 subunit p19; MGC114275; |
Gene ID : | 155140 |
mRNA Refseq : | NM_130410 |
Protein Refseq : | NP_569094 |
Products Types
◆ Recombinant Protein | ||
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Il23a-3516M | Recombinant Mouse Il23a Protein, Myc/DDK-tagged | +Inquiry |
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IL23A-2067R | Recombinant Rhesus Macaque IL23A Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
IL23A-5231HCL | Recombinant Human IL23A 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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