Species : |
Human |
Source : |
HEK293 |
Protein Length : |
Asp 23 - Asp 126 |
Appearance : |
Powder mixture |
Particle size : |
2 μm |
Bio-activity : |
Immobilized 4.1 μg CD3E/1mg beads can bind the Anti Human CD3 Antibody with an EC50 of 0.9022 μg/mL. |
Coupled amount of protein : |
≈265 pmol (4.1 μg) CD3E/mg Beads |
Capacity : |
>2265 pmol (35μg) Anti Human CD3/mg beads |
Formulation : |
PBS, pH7.4, with 10% Trehalose |
Reconstitution : |
2 mL sterile deionized water (1 mg beads/mL) |
Background : |
The Human CD3E-coupled Magnetic Beads is produced by coupling biotinylated CD3E protein to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-CD3E antibody from your sample with high specificity. |
Application : |
This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile. |
Storage : |
Upon receipt, please store the Beads at -20°C for 1 year in lyophilized state. |