Active CD3E protein-coupled magnetic Beads

• Cat.No.: CD3E-0876M

Specification

• Species: Human
• Appearance: Powder mixture
• Particle size: 2 μm
• Source: HEK293
• Protein Length: Asp 23 - Asp 126
• Bio-activity: Immobilized 4.1 μg CD3E/1mg beads can bind the Anti Human CD3 Antibody with an EC50 of 0.9022 μg/mL.
• Coupled amount of protein: ≈265 pmol (4.1 μg) CD3E/mg Beads
• Capacity: >2265 pmol (35μg) Anti Human CD3/mg beads
• Formulation: PBS, pH7.4, with 10% Trehalose
• Reconstitution: 2 mL sterile deionized water (1 mg beads/mL)
• Background: The Human CD3E-coupled Magnetic Beads is produced by coupling biotinylated CD3E protein to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-CD3E antibody from your sample with high specificity.
• Application: This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
• Storage: Upon receipt, please store the Beads at -20°C for 1 year in lyophilized state.