Active EGFRvIII protein-coupled magnetic Beads

• Cat.No.: EGFRvIII-342M

Specification

• Species: Human
• Appearance: Powder mixture
• Particle size: 2 μm
• Source: HEK293
• Protein Length: Leu 25- Ser 378
• Bio-activity: Immobilized 44 μg EGFRvIII protein/1 mg beads can bind Anti-EGFR Antibody (Cetuximab) with an EC50 of 0.7172 μg/mL.
• Coupled amount of protein: ≈261 pmol (11 μg) EGFRvIII/mg beads
• Capacity: >200 pmol (30 μg) antibody/mg beads
• Formulation: PBS, pH7.4, with 10% Trehalose
• Reconstitution: 2 mL sterile deionized water (1 mg beads/mL)
• Background: The Human EGFRvIII-coupled Magnetic Beads is produced by coupling biotinylated EGFRvIII protein to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-EGFRvIII antibody from your sample with high specificity.
• Application: This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
• Storage: Upon receipt, please store the Beads at -20°C for 1 year in lyophilized state.