Active Native Vicia Villosa Lectin Protein, Biotinylated

• Cat.No.: Lectin-1856V
• Product Overview: This product is the Agarose bound Vicia Villosa Lectin and has sugar specificity against N-Acetylgalactosamine.

Specification

• Species: Vicia Villosa
• Source: Vicia Villosa
• Tag: Non
• Description: This lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I. The dominant isolectins in our preparations appear to be B subunit-rich. VVL recognizes preferentially α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen). Evidence suggests that this lectin also may require specific amino acid sequences at the receptor site of glycosylation. The disaccharide galactosyl (α-1,3) N-acetylgalactosamine is also a potent inhibitor of this lectin.
• Form: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM Ca2+, 0.01 mM Mn2+.
• Bio-activity: Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine
• Molecular Mass: 102 kDa - 144 kDa
• Applications: Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
• Usage: For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml.
• Storage: Refrigerate; for long term storage, aliquots may be stored frozen.
• Concentration: 2 mg/ml

Gene Information

• Synonyms: Lectin; VVL; VVA
• UniProt ID: P56625