Active Native Wheat Triticum vulgaris Lectin (WGA), Cy5-Conjugated

• Cat.No.: WGA-240W
• Product Overview: Native Wheat Triticum vulgaris Lectin (WGA) - Cy5
  Blood Group Specificity: Non-specific
  Preferred Sugar Specificity: N-Acetylglucosamine
  Inhibiting or Eluting Sugar: Chitin Hydrolysate or N-Acetylglucosamine w/acid or salt
  Divalent Ions: Ca++
  Excitation: 650 nm
  Emission: 670 nm
  Mitogenic Activity: Yes
  Hazardous Shipping: Non-hazardous
  Fluorescence: Red
  Carbohydrate Specificity: N-Acetylglucosamine

Specification

• Species: Wheat
• Source: Wheat
• Description: Wheat germ agglutinin (WGA) is a lectin that is affinity purified from Triticum vulgaris and non-enzymatically binds to N-acetyl-D-glucosamine and sialic acid residues of glycoproteins and glycolipids. This lectin protects Triticum vulgaris from insects, yeast and bacteria. WGA consists of two subunits and has a molecular weight of 36 kDa. It is an acidic protein and has mitogenic activity toward lymphocytes. It agglutinates erythrocytes and most types of malignant cells. When bound to Cy5, the binding pattern of WGA in cellular imaging and flow cytometry is evident. The excitation wavelength required for Cy5 to fluoresce is high enough to avoid overlap with most other fluorochromes, making it useful for dual-labeling experiments. Because of this high excitation, there is typically less background from autofluorescence of biological specimens.
• Form: Liquid
• Bio-activity: 10 μg/ml will agglutinate human erythrocytes nonspecifically.
• Stability: Shelf Life: 1 year
• Storage: Store frozen at -20 centigrade in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8 centigrade for short term use.
• Concentration: 1 mg/mL

Gene Information

• Official Symbol: WGA
• Synonyms: Triticum vulgaris Lectin; WGA