Active Recombinant C. difficile toxB, His-tagged

• Cat.No.: toxB-12C
• Product Overview: Recombinant C. difficile toxB (Met1-Leu543), fused with a C-terminal 6-His tag, was expressed in E. coli.

Specification

• Species: C.difficile
• Source: E.coli
• Tag: His
• Protein Length: 1-543 a.a.
• Description: Clostridium difficile toxin B is a toxin produced by the bacteria Clostridium difficile. C. difficile produces two major kinds of toxins that are very potent and lethal; an enterotoxin (Toxin A) and a cytotoxin (Toxin B, this protein).
• Predicted N Terminal: Ser2
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to hydrolyze UDP-Glucose.
• Molecular Mass: Predicted Molecular Mass: 64 kDaSDS-PAGE: 60-65 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 °C as supplied.3 months, -20 to -70 °C under sterile conditions after opening.