Active Recombinant Canine FCGRT&B2M protein, His-tagged

• Cat.No.: FCGRT&B2M-1418C
• Product Overview: Recombinant Canine FCGRT&B2M protein(Ala23-Pro298(FCGRT)&Val28-Asn125(B2M)), fused with His tag, was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: His
• Protein Length: 23-298;28-125 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free captured on CM5 chip via anti-His antibody can bind Herceptin with an affinity constant of 0.171 μM as determined in a SPR assay (Biacore 8K).Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free captured on CM5 chip via anti-His antibody can bind Ipilimumab with an affinity constant of 0.132 μM as determined in a SPR assay (Biacore 8K).Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free captured on CM5 chip via anti-His antibody can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with an affinity constant of 1.11 μM as determined in a SPR assay (Biacore 8K).
• Molecular Mass: Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free, produced by co-expression of FCGRT and B2M, has a calculated MW of 32.3 kDa (FCGRT) and 11.6 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 35-37 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.
• Proteinlength: Ala23-Pro298(FCGRT)&Val28-Asn125(B2M)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.