Active Recombinant Canine FCGRT&B2M protein, His-tagged
Cat.No. : | FCGRT&B2M-1418C |
Product Overview : | Recombinant Canine FCGRT&B2M protein(Ala23-Pro298(FCGRT)&Val28-Asn125(B2M)), fused with His tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Canine |
Tag : | His |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
Bio-activity : | Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free captured on CM5 chip via anti-His antibody can bind Herceptin with an affinity constant of 0.171 μM as determined in a SPR assay (Biacore 8K).Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free captured on CM5 chip via anti-His antibody can bind Ipilimumab with an affinity constant of 0.132 μM as determined in a SPR assay (Biacore 8K).Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free captured on CM5 chip via anti-His antibody can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with an affinity constant of 1.11 μM as determined in a SPR assay (Biacore 8K). |
Molecular Mass : | Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free, produced by co-expression of FCGRT and B2M, has a calculated MW of 32.3 kDa (FCGRT) and 11.6 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 35-37 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation. |
Proteinlength : | Ala23-Pro298(FCGRT)&Val28-Asn125(B2M) |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Products Types
◆ Recombinant Protein | ||
Fcgrt-3188M | Recombinant Mouse Fcgrt Protein, His (Fc)-Avi-tagged | +Inquiry |
FCGRT-265C | Recombinant Cynomolgus Monkey FCGRT Protein, His (Fc)-Avi-tagged | +Inquiry |
FCGRT-3998H | Recombinant Human FCGRT Protein, GST-tagged | +Inquiry |
FCGRT-1963R | Recombinant Rat FCGRT Protein, His (Fc)-Avi-tagged | +Inquiry |
FCGRT-902H | Recombinant Human FCGRT Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
FCGRT-6278HCL | Recombinant Human FCGRT 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Ask a questionCustomer Reviews (3)
Write a reviewWith a long shelf life, a single bottle of the reagent can be used multiple times, which is especially valuable for budget-conscious laboratories.
It maintains its activity and effectiveness under various environmental conditions.
The reagent has a wide range of applications, catering to diverse needs in different laboratories, be it in biomedical research or the field of bioengineering.
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