Active Recombinant Canine ITGA11&ITGB1 protein, His-tagged

• Cat.No.: ITGA11&ITGB1-0203C
• Product Overview: Recombinant Canine ITGA11&ITGB1 protein(Asp21-Pro1142(ITGA11)&Asp23-Asp728(ITGB1)), fused with His tag, was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: His
• Protein Length: 21-1142;23-728 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Native Human Collagen I protein at 10 μg/mL (100 μL/well) can bind Canine ITGA11&ITGB1 Heterodimer Protein, His Tag&Tag Free with a linear range of 0.078-5 μg/mL.Immobilized Native Human Collagen IV Protein at 10 μg/mL (100 μL/well) can bind Canine ITGA11&ITGB1 Heterodimer Protein, His Tag&Tag Free with a linear range of 0.01-0.313 μg/mL.
• Molecular Mass: Subunit ITGA1 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus.
• Proteinlength: Asp21-Pro1142(ITGA11)&Asp23-Asp728(ITGB1)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Official Symbol: ITGA11