Active Recombinant Canine MET Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: MET-1037CAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant extracellular domain of canine MET (NP_001002963.1) (Met 1-Leu 935) was expressed, with a polyhistidine tag at the N-terminus.

Specification

• Source: HEK293
• Species: Dog
• Tag: His
• Form: Lyophilized
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.
  2. Immobilized human HGF at 10 μg/mL (100 μL/well) can bind canine c-MET, The EC50 of canine c-MET is 7 ng/mL.
  3. Immobilized canine MET-His at 10 μg/mL (100 μL/well) can bind biotinylated human HG
• Molecular Mass: The recombinant canine c-Met is a disulfide-linked heterodimer composed of proteolytically cleaved α and β subunits. Each α and β subunit together consists of 922 amino acids and has a predicted molecular mass of 103 (α=33 + β=70) kDa. As a result of glycosylation, the apparent molecular mass of the canine c-Met is approximately 42-47 kDa and 85-95 kDa respectively in SDS-PAGE under reducing conditions.
• Protein length: 992
• N-terminal Sequence Analysis: Glu 25
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: MET MET proto-oncogene, receptor tyrosine kinase [ Canis lupus familiaris ]
• Official Symbol: MET
• Synonyms: c-Met; HGF receptor; HGF/SF receptor; SF receptor; met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met
• Gene ID: 403438
• mRNA Refseq: NM_001002963
• Protein Refseq: NP_001002963
• UniProt ID: Q75ZY9