Active Recombinant Chicken NTN1 Protein, His-tagged

• Cat.No.: NTN1-01C
• Product Overview: Recombinant chicken Netrin-1 (Gly26 - Ala606) protein with a 10-His tag was expressed in Mouse myeloma cell line.

Specification

• Description: Netrins control guidance of CNS commissural axons and peripheral motor axons. Promotes neurite outgrowth from commissural axons but acts as a chemorepellent for trochlear motor axons. These effects are mediated by distinct receptors.
• Source: Mouse myeloma cell line
• Species: Chicken
• Tag: His
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized rrUNC5H2/Fc Chimera at 5 μg/mL (100 μL/well) can bind rcNetrin-1 with a linear range of 6-400 ng/mL.
• Molecular Mass: 75-85 kDa
• N-terminal Sequence Analysis: Gly26
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Reconstitution: Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: NTN1 netrin 1 [ Gallus gallus (chicken) ]
• Official Symbol: NTN1
• Synonyms: NTN1; netrin 1; netrin-1
• Gene ID: 396389
• mRNA Refseq: NM_205419
• Protein Refseq: NP_990750
• UniProt ID: Q90922