Active Recombinant Clostridium perfringens α2-3,6-Neuraminidase

• Cat.No.: Neuraminidase-014C
• Product Overview: Recombinant, Clostridium perfringens α2-3,6-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal α2,3 and α2,6 unbranched sialic acid residues from complex carbohydrates and glycoproteins. This enzyme does not exhibit activity on α2,8 or branched sialic acids.

Specification

• Species: Clostridium perfringens
• Source: E.coli
• Tag: Non
• Form: Liquid
• CAS Number: 9001-67-6
• Enzyme Commission number: 3.2.1.18 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.54
• Foreign activity: N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected
• Bio-activity: ≥10 units/mL
  ≥250 units/mg protein
• Unit Definition: One unit is defined as the amount of enzyme that will catalyze the release of 1 μmol of methylumbelliferone from 2''-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic Acid per min at 37 centigrade, pH 5.0.
• Applications: Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0
• Notes: Toxicity: Harmful (C)
• Storage: 2-8 centigrade, do not freeze
• Storage Buffer: In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
• Shipping: Wet ice

Gene Information

• Official Symbol: Neuraminidase
• Synonyms: α2-3,6-Neuraminidase; Clostridium perfringens; Recombinant, E. coli, Acetylneuraminyl Hydrolase; Sialidase