| Source : |
HEK293 |
| Species : |
COVID-19 |
| Tag : |
His-Avi |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
| Bio-activity : |
Immobilized Biotinylated SARS-CoV-2 Spike NTD, His,Avitag (BA.4 & BA.5/Omicron) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb, Human IgG1 with a linear range of 0.1-2 ng/mL. |
| Molecular Mass : |
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag)The protein has a calculated MW of 34.4 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein Length : |
Ser 13 - Leu 303 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
| Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
