Active Recombinant COVID-19 Spike protein RBD (K417T, E484K, N501Y), Fc-tagged
Cat.No. : | Spike-4714V |
Product Overview : | Recombinant COVID-19 protein RBD (K417T, E484K, N501Y)(Arg 319 - Lys 537), fused with Fc tag, was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | COVID-19 |
Tag : | Fc |
Form : | Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), Fc Tag at 2 μg/mL (100 μL/well) can bind Human ACE2, His Tag with a linear range of 39-156 ng/mL.Immobilized Human ACE2, His Tag at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), Fc Tag with a linear range of 0.1-0.8 ng/mL. |
Molecular Mass : | This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 51.1 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein Length : | Arg 319 - Lys 537 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] |
Official Symbol : | Spike |
Synonyms : | Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron |
Gene ID : | 43740568 |
Protein Refseq : | YP_009724390.1 |
UniProt ID : | P0DTC2 |
Products Types
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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