Active Recombinant COVID-19 Spike protein S1 subunit, His-tagged

Cat.No. : Spike-191V
Product Overview : Recombinant COVID-19 Spike protein S1 subunit was fused to His tag at C-terminus and expressed in human cells.
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Source : HEK293
Species : COVID-19
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4
Bio-activity : 1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2/2019-nCoV Spike Protein (S1 Subunit, His tag), the EC50 of SARS-CoV-2/2019-nCoV Spike Protein (S1 Subunit, His tag) is 200-600 ng/mL.
2. Loaded ACE2 Protein, Human, Recombinant (mFc Tag) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein with an affinity constant of 47.7 nM as determined by Octet RED System.
Molecular Mass : The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, His Tag) consists of 681 amino acids and predicts a molecular mass of 76.5 kDa.
Protein Length : Val16-Arg685
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 90 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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