Active Recombinant COVID-19 Spike protein S1 subunit receptor binding domain (RBD), mFc-tagged

• Cat.No.: Spike-19V
• Product Overview: Recombinant COVID-19 Spike protein S1 subunit receptor binding domain (RBD) was fused to Mouse IgG1 Fc tag at C-terminus and expressed in human cells.

Specification

• Species: Sars-CoV-2
• Source: HEK293
• Tag: mFc
• Protein Length: Arg319-Phe541
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag), the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag) is 10-30 ng/mL.
  2. Loaded Human ACE2 Protein (His tag) on SA Biosensor, can bind SARS-CoV-2/2019-nCoV Spike/RBD Protein (RBD, mFc Tag) with an affinity constant of 0.562 nM as determined by Octet RED System.
• Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag) consists of 457 amino acids and predicts a molecular mass of 51.5 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.