Active Recombinant COVID-19 Spike RBD protein(BA.3/Omicron), His-tagged

• Cat.No.: Spike-731V
• Product Overview: Recombinant COVID-19 Spike RBD protein(BA.3/Omicron)(Arg 319 - Lys 537)(Accession # QHD43416.1 (G339D,S371F,S373P,S375F,D405N,K417N,N440K,G446S,S477N,T478K,E484A,Q493R,Q498R,N501Y,Y505H)), fused with His tag at the C-terminus, was expressed in HEK293.

Specification

• Source: HEK293
• Species: COVID-19
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized SARS-CoV-2 Spike RBD, His Tag (BA.3/Omicron) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 1-31 ng/mL.
• Molecular Mass: The protein has a calculated MW of 26.8 kDa. The protein migrates as 33-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Arg 319 - Lys 537
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
  >95% as determined by SEC-MALS.
• Storage: This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: Spike
• Synonyms: Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron
• Gene ID: 43740568
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2