Active Recombinant COVID-19 Spike RBD protein(BA.4&BA.5/Omicron), His-tagged
|Product Overview :||Recombinant COVID-19 Spike RBD protein(BA.4&BA.5/Omicron)(Arg 319 - Lys 537)(Accession # QHD43416.1(G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H)), fused with His tag at the C-terminus, was expressed in HEK293.|
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|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.|
|Bio-activity :||Immobilized Human ACE2, Fc Tag at 5 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD, His Tag (BA.4&BA.5/Omicron) with a linear range of 16-125 ng/mL.|
|Molecular Mass :||The protein has a calculated MW of 26.7 kDa. The protein migrates as 33-36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Arg 319 - Lys 537|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
|Storage :||This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.|
|Gene Name :||S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]|
|Official Symbol :||Spike|
|Synonyms :||Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron|
|Gene ID :||43740568|
|Protein Refseq :||YP_009724390.1|
|UniProt ID :||P0DTC2|
|Spike-1058HCL||Recombinant HCoV-EMC/2012 Spike cell lysate||+Inquiry|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.