Active Recombinant COVID-19 Spike RBD protein(BA.4&BA.5/Omicron), His-tagged

• Cat.No.: Spike-710V
• Product Overview: Recombinant COVID-19 Spike RBD protein(BA.4&BA.5/Omicron)(Arg 319 - Lys 537)(Accession # QHD43416.1(G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H)), fused with His tag at the C-terminus, was expressed in HEK293.

Specification

• Species: Sars-CoV-2
• Source: HEK293
• Tag: His
• Protein Length: Arg 319 - Lys 537
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human ACE2, Fc Tag at 5 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD, His Tag (BA.4&BA.5/Omicron) with a linear range of 16-125 ng/mL.
• Molecular Mass: The protein has a calculated MW of 26.7 kDa. The protein migrates as 33-36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
  >90% as determined by SEC-MALS.
• Storage: This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: Spike
• Synonyms: Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron
• Gene ID: 43740568
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2