Source : |
HEK293 |
Species : |
COVID-19 |
Tag : |
His |
Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized SARS-CoV-2 Spike RBD (L452R, T478K), His Tag at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 0.2-3 ng/mL.Immobilized SARS-CoV-2 Spike RBD (L452R, T478K), His Tag at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 with a linear range of 0.1-1 ng/mL.Immobilized Human ACE2, Fc Tag at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (L452R, T478K), His Tag with a linear range of 20-78 ng/mL.Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (L452R, T478K), His Tag with a linear range of 0.3-3 ng/mL. |
Molecular Mass : |
This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 26.6 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein Length : |
Arg 319 - Lys 537 |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>90% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:-20°C to -70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |