Active Recombinant COVID-19 Spike RBD Protein (R346S, N394S, Y449N, E484K, F490S, N501Y), His-Avi-tagged, Biotinylated

Cat.No. : Spike-4650V
Product Overview : Biotinylated Recombinant COVID-19 Protein (R346S, N394S, Y449N, E484K, F490S, N501Y)(Arg 319 - Lys 537), fused with His and Avi tag, was expressed in HEK293.
  • Specification
  • Gene Information
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Source : HEK293
Species : COVID-19
Tag : His-Avi
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Biotinylated SARS-CoV-2 Spike RBD (R346S, N394S, Y449N, E484K, F490S, N501Y), His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human ACE2, Fc Tag with a linear range of 0.1-6 ng/mL.Immobilized Biotinylated SARS-CoV-2 Spike RBD (R346S, N394S, Y449N, E484K, F490S, N501Y), His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 with a linear range of 0.5-3 ng/mL.Immobilized Biotinylated SARS-CoV-2 Spike RBD (R346S, N394S, Y449N, E484K, F490S, N501Y), His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Mouse IgG1 (AM122) with a linear range of 0.1-3 ng/mL.
Molecular Mass : This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 28.1 kDa. The protein migrates as 33-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein Length : Arg 319 - Lys 537
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
Official Symbol : Spike
Synonyms : Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron
Gene ID : 43740568
Protein Refseq : YP_009724390.1
UniProt ID : P0DTC2

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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