Active Recombinant COVID-19 Spike RBD Protein (S477N, E484K), His-Avi-tagged, Biotinylated
Cat.No. : | Spike-4703V |
Product Overview : | Biotinylated Recombinant COVID-19 Protein (S477N, E484K)(Arg 319 - Lys 537), fused with His and Avi tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | COVID-19 |
Tag : | His&Avi |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
Bio-activity : | Immobilized Biotinylated SARS-CoV-2 Spike RBD (S477N, E484K), His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human ACE2, Fc Tag with a linear range of 0.2-2 ng/mL.Immobilized Biotinylated SARS-CoV-2 Spike RBD (S477N, E484K), His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 with a linear range of 0.1-2 ng/mL. |
Molecular Mass : | This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag)The protein has a calculated MW of 28.2 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein length : | Arg 319 - Lys 537 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] |
Official Symbol : | Spike |
Synonyms : | Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron |
Gene ID : | 43740568 |
Protein Refseq : | YP_009724390.1 |
UniProt ID : | P0DTC2 |
Products Types
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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