Active Recombinant Cynomolgus CD3E & CD3D protein, His &Flag-tagged

• Cat.No.: CD3E & CD3D-2974C
• Product Overview: Recombinant Cynomolgus CD3E&CD3D protein(Gln 22-Asp 117 (CD3E) & Phe 22-Ala 105 (CD3D)), fused with His and Flag tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Flag&His
• Protein Length: Gln 22-Asp 117 (CD3E) & Phe 22-Ala 105 (CD3D)
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag & Flag Tag at 2 μg/mL, add increasing concentrations of Bispecific T cell Engager (CD3 X BCMA) and then add Biotinylated BCMA Fc,Avitag at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.2 ng/mL.
• Molecular Mass: Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (MALS verified) is produced by co-expression of CD3E and CD3D, has a calculated MW of 15.5 kDa (CD3E) and 14.5 kDa (CD3D). Subunit CD3E is fused with his tag at the C-terminus and subunit CD3D is fused with flag tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) and Phe 22 (CD3D). The reducing (R) heterodimer protein migrates as 19-22 kDa and 25-40 kDa due to glyosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.