Active Recombinant Cynomolgus HHLA2 protein, His-tagged

• Cat.No.: HHLA2-2898C
• Product Overview: Recombinant Cynomolgus HHLA2 protein(Ile 21-Asn 345), fused with His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: Ile 21-Asn 345
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Loaded Cynomolgus B7-H7, His Tag on HIS1K Biosensor, can bind Human CD28H, Fc Tag with an affinity constant of 13.3 nM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 39.2 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.