| Source : |
HEK293 |
| Species : |
Cynomolgus |
| Tag : |
His |
| Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Cynomolgus ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGF-Beta 1, His,Avitag with a linear range of 0.5-16 ng/mL. |
| Molecular Mass : |
Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 31 (ITGAV) & Glu 43 (ITGB8). The non-reducing (NR) protein migrates as 135-150 kDa (ITGAV) & 75-85 kDa (ITGB8) respectively due to glycosylation. |
| Proteinlength : |
Phe31-Pro993(ITGAV)&Glu43-Ser681(ITGB8) |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Official Symbol : |
ITGAV |
