Active Recombinant Cynomolgus ITGAV&ITGB8 protein, His-tagged

• Cat.No.: ITGAV&ITGB8-0167C
• Product Overview: Recombinant Cynomolgus ITGAV&ITGB8 protein(Phe31-Pro993(ITGAV)&Glu43-Ser681(ITGB8)), fused with His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: 31-993;43-681 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGF-Beta 1, His,Avitag with a linear range of 0.5-16 ng/mL.
• Molecular Mass: Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 31 (ITGAV) & Glu 43 (ITGB8). The non-reducing (NR) protein migrates as 135-150 kDa (ITGAV) & 75-85 kDa (ITGB8) respectively due to glycosylation.
• Proteinlength: Phe31-Pro993(ITGAV)&Glu43-Ser681(ITGB8)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Official Symbol: ITGAV