Active Recombinant Cynomolgus KLK3 protein, His-tagged, Low Endotoxin

• Cat.No.: KLK3-4569C
• Product Overview: Recombinant Cynomolgus KLK3 protein(Q6DT45)(25-261 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: 25-261 aa
• Form: Lyophilized from 0.22 μm filtered solution in 50mM Tris, 150mM NaCl, 8% trehalose, 0.05%Brij-35 (pH 7.5).
• Bio-activity: Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-RPY-pNA). The specific activity is >100 pmol/min/μg.
• Molecular Mass: The protein has a predicted MW of 27.76 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.